 Hold the Steam
Sterilization in laboratory animal facilities has recently seen several changes aimed at decreasing costs; increasing workplace safety and comfort; and minimizing wear on cages and equipment.
Traditional steam sterilizing processes use an autoclave - a pressurized device used to transfer heat in order to sterilize the contents. In order for the device to work properly, the autoclave must not be overcrowded, and the lids of bottles and containers must be ajar. Also, the autoclave bears initial cost, operating cost (steam boiler, distribution lines, high volumes of water, and licensing of operators), and maintenance costs.
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